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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become noticed in several tumors, which results in chromosome instability, and so may perhaps lead to tumorigenesis. This protein was discovered to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant purpose in selling cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two added splice variants had been documented but specific nucleotide sequence details is not really however readily available.
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Blocking peptides are peptides that bind especially for the target antibody and block antibody binding. These peptide normally consists of the epitope identified by the antibody.
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This manual outlines further doable optimization of mobile and immunoassay parameters to make certain the best possible success are...
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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
Antibodies sure to the blocking peptide now not bind to your epitope over the concentrate on protein. By evaluating the staining from the blocked antibody compared to the antibody alone, one can see which staining is unique.
Concentrate on Information Cyclin E belongs to the really conserved cyclin family members, whose members are characterised by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins purpose as regulators of CDK kinases. Unique cyclins exhibit unique expression and degradation styles which contribute to your temporal coordination of each and every mitotic celebration. Cyclin E forms a fancy with and functions being a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and it is degraded as cells development by S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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